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Publications

  • Mazuz, M. L., T.  Molad, L. Fish, B. Leibovitz, R. Wolkomirsky, L. Fleiderovitz and V. Shkap (2012). Genetic diversity of Babesia bovis in virulent and attenuated strains. Parasitology, 139(3), 317-323.

  • Erster,O.,A. Roth, R. Wolkomirsky, B. Leibovich, V. Shkap (2013). Comparative analysis of mitochondrial markers from four species of Rhipicephalus (Acari: Ixodidae). Vet Parasitol. Dec 6;198(3-4):364-70.

  • Erster O., Roth A. and Shkap V. (2013). First detection of Ixodes ricinus on beef cattle in Israel.Vet Parasitol. Jan 31;191(3-4):394-9

  • Shimshoni,  J.,Erster,  O., Rot, A., Cuneah, O., Soback, S., Shkap V.(2013). Cuticular fatty acid profile analysis of three Rhipicephalus tick species (Acari: Ixodidae).Exp. Appl. Acarology, 61(4), 481-489.

  • Erster O., Roth A., Leibovich B., Wolkomirsky R., Savitzky I. and Shkap V. Efficacy of Babesia ovis transmission by different tick stages or blood merozoites. Manuscript in preparation.

  • S. Renneker, J. Abdo, M.A. Bakheit, B. Kullmann, D. Beyer, J. Ahmed and U. Seitzer: Coinfection of Sheep with Anaplasma, Theileria and Babesia Species in the Kurdistan Region, Iraq (pages 113–118) Article first published online: 8 NOV 2013 | DOI: 10.1111/tbed.12148

  • D. A. Flores, Y. Minichiello, F. R. Araujo, V. Shkap, D. Benítez, I. Echaide, P. Rolls, J. Mosqueda, G. M. Pacheco, M. Petterson, M. Florin-Christensen and L. Schnittger:  Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation (pages 131–136) Article first published online: 8 NOV 2013 | DOI: 10.1111/tbed.12121

  • S. B. Mohammed, M. A. Bakheit, M. Ernst, J. S. Ahmed and U. Seitzer: Identification and Characterization of Theileria annulata Heat-Shock Protein 90 (HSP90) Isoforms (pages 137–149). Article first published online: 8 NOV 2013 | DOI: 10.1111/tbed.12150

  • Rodriguez, A.E., Schnittger, L., Tomazic, M., Florin-Christensen, M. Current and prospective tools for the control of cattle-infecting Babesia parasites. In: “Protozoa: Biology, Classification and Role in Disease” (Castillo, V. & Harris R., eds). Nova Science Publishers, Hauppauge NY, USA, 2012.

  • Rodriguez, A., Zamorano, P., Wilkowsky, S., Torrá, F., Ferreri, L., Dominguez, M., Wilkowsky, S., Florin-Christensen, M. A Delivery of recombinant vaccines against bovine herpesvirus type 1 gD and Babesia bovis MSA-2c to mice using liposomes derived from egg yolk lipids. The Veterinary Journal, 2012 Nov 23. doi:pii: S1090-0233(12)00471-6. 10.1016/j.tvjl.2012.10.036. [Epub ahead of print]

  • Schnittger, L., Rodriguez, A., Florin-Christensen, M., Morrison, D.A.. Babesia: a world emerging. Infection, Genetics and Evolution 12: 1788-1809, 2012

  • Flores, D., Minichiello, Y., Araujo, F.R., Shkap, V., Benítez, D., Echaide, I., Rolls, P., Mosqueda, J., Pacheco, G.M., Petterson, M., Florin-Christensen, M., Schnittger, L. Evidence for extensive genetic diversity and sub-structuring of the Babesia bovis metapopulation. 2013, submitted.

  • Niu Q, Bonsergent C, Guan G, Yin H, Malandrin L. Sequence and organization of the rhoptry-associated-protein-1 (rap-1) locus for the sheep hemoprotozoan Babesia sp. BQ1 Lintan (B. motasi phylogenetic group). Vet Parasitol. 2013 Nov 15;198(1-2):24-38.

  • Mohamed Ridha Rjeibi, Mohamed Gharbi1,, Moez Mhadhbi, Wiem Mabrouk, Boutheïna Ayari, Ines Nasfi, Mohamed Jedidi, Limam Sassi, Mourad Rekik and Mohamed Aziz Darghouth
    Prevalence of piroplasms in small ruminants in northwest Tunisia and first genetic characterization of Babesia ovis in Africa Accepted in Parasite

  • Jouglin M, Fernandez-de-Mera IG, De la Cotte N, Ruiz-Fons F, Bastian S., Gortazar C, Moreau E, de la Fuente J, Malandrin L. Isolation and characterization of Babesia pecorum sp. nov. from farmed red deer (Cervus elaphus). Vet Res Revision 2

  • Niu Q, Marchand J, Cogshan Y, Bonsergent C, Guan G, Yin H, Malandrin L. Rhoptry-associated-Protein (rap-1) genes in the sheep pathogen Babesia sp. Xinjiang : multiple copies differing by 3' end repeated sequences. PloSone Revision 1


Submitted and under preparation

  • Niu Q, Valentin C, Bonsergent C, Malandrin L. Strong conservation of rhoptry-associated-protein-1 (RAP-1) locus organization and sequence amond Babesia isolates infecting sheep from China (Babesia motasi-like phylogenetic group). Submitted to Parasitol Res

  • Niu Q, Bonsergent C, Guan G, Malandrin L, Moreau E. Expression of rap-1 genes in Babesia sp. BQ1 (Lintan) (B. motasi-like phylogenetic group), a pathogen of sheep in China.

  • Mohamed Ridha Rjeibi, Mohamed Gharbi, Mourad Rekik, Brahim Amor, Limam Sassi1 and Mohamed Aziz Darghouth
    First molecular identification and genetic characterization of Theileria lestoquardi in sheep of the Maghreb region Minor revision in Transboundary and Emerging Diseases

  • Mohamed Gharbi, Nadhem Sakly, Mohamed Aziz Darghouth Prevalence of Dermanyssus gallinae (Mesostigmata: Dermanyssidae) in industrial poultry farms in North-East Tunisia. Parasite, In Press, 2014

  • First molecular identification and genetic characterization of Theileria lestoquardi in sheep of the Maghreb region
    Transboundary & Emerging Disease Under revision


PhD thesis

  • Niu Q. Comparison of sequences, organisation and expression of the rhoptry-associated-protein-1 genes in Babesia isolates responsible for ovine babesiosis in China, 2014 at Oniris.


Selected oral presentations and other communications

  • Orrá, F., Rodriguez, A.E., Echaide, I., Schnittger, L., Florin-Christensen, M. Glycosylphosphatidylinositol of bovine Babesia parasites (oral communication) Joint Conference on Emerging and re-emerging epidemics affecting global health 2012, Orvieto, Italy, September 20 2012

  • Mesplet, M.; Torrá, F., Florin-Christensen, M.; Schnittger, L. Peptidasas en piroplasmas bovinos patógenos: Análisis genómico comparativo entre Babesia bovis y Theileria annulata (oral communication). VI Congreso Argentino de Parasitología, Bahia Blanca,  Argentina, October 17, 2012

  • Flores, D., Minichiello, Y., Araujo, F.R., Shlap, V., Benítez, D., Echaide, I., Rolls, P., Mosqueda, J., Pacheco, G.M., Petterson, M., Florin-Christensen, M., Schnittger, L. Typification of Babesia bovis reference isolates using micro and minisatellite markers. Joint Conference on Emerging and re-emerging epidemics affecting global health 2012, Orvieto, Italy, 19-22 September, 2012. (Poster)

  • Schnittger, L., invited speaker (Spanish): Desarrollo y aplicación de micro y minisatelites para la tipificacion multilocus de Babesia bovis de variados origenes geograficos. (Development and application of micro and minisatellites for the multilocus typification of Babesia bovis of varied geographic origins) XXV Meeting of the Argentine Society of Protozoology, Buenos Aires, Argentina, 30 August, 2012.

  • Schnittger, L., short oral communication (English): Evolutionary history, molecular phylogeny and taxonomy of babesia parasites. Genomics, Proteomics and Transcriptomics session, Joint Conference on Emerging and re-emerging epidemics affecting global health 2012, Orvieto, Italy, 22 September, 2012.

  • Schnittger, L., invited speaker (Spanish): Historia evolutiva y filogenia molecular del género Babesia sp. (Evolutionary history and molecular phylogeny of the Babesia sp. genus) VI Argentine Congress of Parasitology, Bahia Blanca, Argentina, 17 October 2012.

  • Niu Q, Bonsergent C, Valentin C, Moreau E, Guan G, Yin H, Malandrin L. 2013. The Rhoptry-Associated-Protein 1 locus in the sheep parasite Babesia sp. BQ1 (Lintan) Many conserved features with B. bigemina. 2nd International Meeting on ApicoWplexan parasites in farm animals. 31 Oct-2 Nov, Kusadasi, Turkey.

  • Niu Q, Bonsergent C, Moreau E, Guan G, Yin H, Malandrin L. 2012. Babesia sp. BQ1 Lintan (B. motasi group) rap-1 locus. A target to vaccine development. Joint Conference on Emerging and Re-emerging Epidemics Affecting Global Health, 19-22 Sept, Orvietto, Italy.

  • Niu Q, Marchand J, Bonsergent C, Congshan Y, Guan G, Yin H, Malandrin L. 2013. The rhoptry-associated-protein 1 family in the sheep parasite Babesia sp. Xinjiang. 2013. 2nd International Meeting on ApicoWplexan parasites in farm animals. 31 Oct-2 Nov, Kusadasi, Turkey. (Poster)

  • Niu Q, Marchand J, Bonsergent C, Guan G, Yin H, Moreau E, Malandrin L. Babesiosis of small ruminants: Evaluation of Rhoptry-Associated-Protein-1 (RAP-1) as a vaccine candidate. Nov 2012. Meeting of the French group “Tick and Tick-transmitted diseases”, Clermont-Ferrand, France.

  • Niu Q, Moreau E, Chauvin A, Malandrin L. Babesiosis of small ruminants: evaluation of the Rhoptry-associated-protein RAP-1 as a vaccine candidate. Nov 2011. Talmont St Hilaire, France (Poster)

  • Hüseyin Bilgin Bilgiç, Ahmet Hakan Ünlü, Onur Köse, Serkan Bak?rc?, Selin
    Hac?larl?o?lu, Hasan Eren, Tülin Karagenç. Koyun ve Keçilerde.  Theileria/Babesia ve Ehrlichia/Anaplasma Türlerinin Belirlenmesi ( Identification of Theileria/ Babesia ve Ehrlichia/Anaplasmaspecies in Sheep and Goats) . 18.National Parasitology Congress, 29 September- 5 October 2013.

  • Bilgiç H, Ünlü H, Bak?rc? S, Hac?larl?o?lu S, Köse O, Eren H. Karagenç T (2013). Prevalence of Theileria/Babesia and Anaplasma/Ehrlichia species in small ruminants in Turkey and diagnostic sensitivity of single-PCR and RLB, 2nd ApiCOWplexa Apicomplexa in farm animals, International meeting, Kusadasi, Ayd?n, Türkiye (Sözlü bildiri), 31 October-2 November, 2013

  • Mohammed Bakheit,  Hyalomma ticks and their role as carriers of disease agents. Second Workshop on Tick-Borne Diseases. 27. -28. June 2013, Berlin, Germany

  • Mackiewicz, Monika, Host-pathogen interactions – the rising impact of posttranslational modifications. Second Workshop on Tick-Borne Diseases. 27. -28. June 2013, Berlin, Germany

  • Tschitschmann, Susan,  The role of histone deacetylases as potential trigger for attenuation and stage differentiation of Theileria. Second Workshop on Tick-Borne Diseases. 27. -28. June 2013, Berlin, Germany


Activities developed by IBET

Babesia ovis prevalence in Portugal

Resorting for Babesia and Theileria ovis diagnostic, blood and serum samples from small ruminants were collected from different regions of Portugal. In the total, more than 600 samples were processed and Polymerase chain reaction (PCR) using specific primers for both species were performed. So far, the estimated prevalence in the country for B. ovis and T. ovis is around 5% and 12%, respectively. Nested-PCR for Babesia and Theileria ovis were developed and higher sensitivity were achieved when compared to the convencional PCR.

Proteome and antigenic proteins of Babesia ovis

Characterization of the Babesia ovis proteome during in vitro infection using a 2D gel electrophoresis with a non-linear pH range 3-10. Three different culture media were choosen: two new serum-free culture media and the usual medium, which is complete medium culture (CM). The immunogenic proteins expressed by the parasite when the erythrocyte is recognized are studied in all the previous described culture media. Three proteins (35, 55, 200 KDa) expressed by the parasite growing in CM and two (35 and 200 KDa) in VyM media cultures show immunogenicity when challenged with the positive sheep serum.

Posters

  • Molecular identification of Babesia and Theileria species infecting small ruminants in Portugal Joana Campos & Abel Oliva. 11th Biennial Conference of the Society for Tropical Veterinary Medicine (STVM) September 19 to 22 in Orvieto, Italy.
  • 2D electrophoresis of the proteins produced by Babesia ovis during infection. Maria do Carmo Barreto, Miguel Ventosa, Isabel Marcelino, S. Horta, Abel Oliva. 2nd International Meeting on Apicomplexan Parasites in Farm Animals. October 31 – November 2, 2013. Kusadasi, Turkey
  • Proteins produced by Babesia ovis during the infection process. Maria do Carmo Barreto, S. Horta, Abel Oliva. 2nd International Meeting on Apicomplexan Parasites in Farm Animals. October 31 – November 2, 2013. Kusadasi, Turkey

Students PhD, MSc

PhD Thesis: Sara Horta has proposed for a PhD fellowship to the FCT (Science and Technology Foundation, Portugal) Thema: “Post-translational modification of glycolytic enzymes and its influence on Theileria-induced leukocyte transformation.”